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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: J Hepatol. 2018 Apr 27;69(3):687–696. doi: 10.1016/j.jhep.2018.04.004

Fig. 5. Hcar2 is expressed on liver macrophages and BHB promoted an M2 macrophage phenotype in the liver.

Fig. 5

(A) Immunofluorescence of Hcar2 expression in the liver. Blue, DAPI; red(mRFP), Hcar2; Green, F4/80 or CD32. (B&C) Wild-type mice were injected i.p. with Etomoxir (20mg/kg) or saline. 30 minutes later they were gavaged with ethanol (6g/kg). Mice were given an i.p. injection of BHB (3mmol/kg body weight) 6 hours after ethanol gavage and livers were collected 10 hours later. (B) Expression of M1 markers on liver macrophages (CD45+CD11b+F4/80+) by flowcytometry. (C) Expression of M2 markers on liver macrophages (CD45+CD11b+F4/80+) by flow cytometry. (Quantified expression values of M1 and M2 markers on macrophage in the liver (n=7 per group). ΔMFI=(Mean Fluoresce Intensity)positive staining – (Mean Fluoresce Intensity)isotype staining. Data were analyzed with nonparametric Wilcoxon ranksum tests.* P<0.05; ** P<0.01.