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One–dimensional analysis of A3 and RCAM A3 unfolding to obtain transition midpoints and unfolding cooperativity. Iso–thermal urea unfolding monitored A) by CD at 222 nm and B) by fluorescence (λEx = 280nm, λEm = 359nm). Iso–urea thermal unfolding at a scan rate of 2°C/min monitored C) by CD at 222 nm and D) by fluorescence (λEx = 280nm, λEm = 359nm).
