Table 2.

Validation and optimization of HPLC

Sr. no.	Validation parameter	Results
1	Linearity range	5–25 ppm
2	Regression equation	Slope	36081
		Intercept	351181
3	Regression coefficient	0.9
4	Precision	%RSD first day	0.6
		%RSD second day	0.1
5	Accuracy (% recovery)	SD	1.2
		%RSD	1.2
		Mean	100.7
6	LOD (μg/ml)	0.6
7	LOQ (μg/ml)	1.7