Fig. 2.

Immunohistochemical microphotographs of tissue factor and thrombus in rabbit normal and atherosclerotic femoral arteries

(Left column) Representative microphotographs of normal femoral artery and of femoral arteries at 3 weeks after balloon injury of conventional (SMC-rich neotima) or 0.5% cholesterol diet (Macrophage-rich neointima). (Middle column) Tissue factor is expressed in SMC- and macrophage-rich neointima, and in adventitia. (Right column) Thrombus 15 minutes after balloon injury on normal artery comprises only small aggregated platelets, whereas that on neointima comprises a mixture of platelets and fibrin. Thrombus on macrophage-rich neointima is much larger. Abbreviations: Ad, adventitia; HE/VB, hematoxylin and eosin/Victoria blue; I, intima; M, media (modified from Ref. 35, with permission).