FIGURE 4.

Effect induced by Sy-HPBCD and Sy-RAMEB complexes on the histological changes in liver of CCl₄-treated mice. (A) H&E. (a) Control group: normal lobular architecture and cell structure; (b) CCl₄-treated group: extensive hepatocellular damage with the presence of inflammatory cell infiltration, steatosis and necrosis, pseudo-lobular formation with collagen deposition; (c) CCl₄-control group: the recovery was still weak and aspect almost similar to CCl₄-treated group; (d) CCl₄ and Sy-RAMEB co-treated group: mild inflammation, minimal hepatocellular necrosis and less extended fibrotic septa; (e) CCl₄ and Sy-HPBCD co-treated group: histological aspect closest to the control with minimal inflammatory cells; (f) CCl₄ and free Sy co-treated group: minimal fibrotic changes are present. ^∗Fibrotic septa and collagen deposition; arrow – inflammatory cell infiltration; scale bar – 50 μm. (B) Fouchet van Gieson trichrome. (a) Control group: no significant collagen deposition; (b) CCl₄-treated group: substantial collagen deposition in the periportal and pericellular areas, large fibrous septa formation, pseudo-lobe separation; (c) CCl₄-control group: the recovery was still weak and aspect almost similar with CCl₄-treated group; (d) CCl₄ and Sy-RAMEB co-treated group: less collagen deposition was observed than that in mice treated with CCl₄ alone; (e) CCl₄ and Sy-HPBCD co-treated group: histological aspect closest to the control; (f) CCl₄ and free Sy co-treated group: fibrotic changes are still present; arrow- fibrotic septa and collagen deposition; scale bar – 50 μm. (C) Histogram showing the percentage area of Fouchet van Gieson staining of collagen. ^∗p < 0.05 compared to control; ^∗∗∗p < 0.001 compared to control; ^###p < 0.001 compared to CCl4 group; ⁺⁺p < 0.01 compared to CCl4 control group; ⁺⁺⁺p < 0.001 compared to CCl4 control group; ˆp < 0.05 compared to CCl4/Sy group.