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. 2018 Aug 13;9:823. doi: 10.3389/fphar.2018.00823

FIGURE 4.

FIGURE 4

Urinary trypsin inhibitor enhanced expression of tight junctional proteins in LPS-treated PMVECs. Confocal images showing expression of tight junctional proteins in PMVECs. Increased occludin (Aa1–3), claudin-5 (Ba1–3), and ZO-1 (Ca1–3) expression (red) was detected in CD31 positive PMVECs (green) of control group. It was hardly detected in LPS group (Ab1–3,Bb1–3,Cb1–3). The expression was markedly enhanced in LPS group that was agiven UTI pretreatment (Ac1–3,Bc1–3,Cc1–3). Western blot shows expression levels of occludin (D), claudin-5 (E), and ZO-1 (F). Note that the expression of all three proteins was significantly decreased in LPS when compared with control (p = 0.000, 0.001, and 0.001, respectively); it was significantly reversed to levels comparable to that of the control when pretreated with UTI (p = 0.000, 0.004, and 0.006, respectively). Scale bars: 50 μm, DAPI-blue. All values represent mean ± SD in triplicate; ∗represent marked differences compared with corresponding control.