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. 2018 Aug 13;9:823. doi: 10.3389/fphar.2018.00823

FIGURE 6.

FIGURE 6

TNF-α receptor antagonist enhanced expression of tight junctional proteins in TNF-α treated PMVECs. Confocal images showing occludin (A), claudin-5 (B), and ZO-1 (C) expression in PMVECs. Increased occludin, claudin-5, and ZO-1 expression (red) was detected in CD31 positive PMVECs (green) of control group (Aa1–3,Ba1–3,Ca1–3). It was hardly detected in TNF-α group (Ab1–3,Bb1–3,Cb1–3). The expression was markedly increased when TNF-α receptor antagonist was given (Ac1–3,Bc1–3,Cc1–3). Western blot shows expression level of occludin (D), claudin-5 (E), and ZO-1 (F) were significantly decreased in TNF-α when compared with control (p = 0.001, 0.000, and 0.001, respectively); it was significantly reversed to levels comparable to that of the control when pretreated with TNF-α receptor antagonist (p = 0.004, 0.003, and 0.002, respectively). Scale bars: 50 μm, DAPI-blue. All values represent mean ± SD in triplicate; ∗represent marked differences compared with corresponding control.