Table 3.
Antibodies used for Western blotting and immunostaining.
| Antibody | Host | Source | Catalog number | Dilution for staining | Dilution for Western blot |
|---|---|---|---|---|---|
| CD31 | Mouse monoclonal | Abcam, United Kingdom | ab64543 | 1:100 | |
| Occludin | Rabbit polyclonal | Thermo Fisher Scientific, United States | 71–1500 | 1:100 | 1:1000 |
| Claudin5 | Mouse monoclonal | Thermo Fisher Scientific, United States | 35–2500 | 1:100 | 1:1000 |
| ZO-1 | Rabbit polyclonal | Thermo Fisher Scientific, United States | 40–2200 | 1:100 | 1:1000 |
| TNF-α | Rabbit polyclonal | Abcam, United Kingdom | Ab6671 | 1:100 | 1:1000 |
| GAPDH | Mouse monoclonal | Biworld | MA001 | 1:3000 | |
| p-P38 | Rabbit monoclonal | Cell Signaling Technology, United States | 4511S | 1:1000 | |
| P38 | Rabbit monoclonal | Cell Signaling Technology, United States | 8690S | 1:1000 | |
| p-Erk | Rabbit monoclonal | Cell Signaling Technology, United States | 4695S | 1:1000 | |
| Erk | Rabbit monoclonal | Cell Signaling Technology, United States | 4370S | 1:1000 | |
| p-JNK | Mouse monoclonal | Cell Signaling Technology, United States | 9255S | 1:1000 | |
| JNK | Mouse monoclonal | R&D Systems, United States | MB2076 | 1:1000 | |
| p-IκB | Goat polyclonal | Santa Cruz Biotechnology, United States | Sc-7977 | 1:1000 | |
| IκB | Mouse monoclonal | Cell Signaling Technology, United States | 4814S | 1:1000 | |
| IgG-HRP | rabbit polyclonal | Cell Signaling Technology, United States | 7074S | 1:3000 | |
| IgG-HRP | Mouse monoclonal | Cell Signaling Technology, United States | 7076S | 1:3000 | |
| Secondary Antibody | Donkey polyclonal | Thermo Fisher Scientific, United States | A-31572 | 1:200 | |
| Secondary Antibody | Donkey polyclonal | Thermo Fisher Scientific, United States | A-21202 | 1:200 |