Table 2.
Effect of extracts and compounds 1-9 on Cav2.2 N-type VGCC.
| Fraction | Cav2.2a (N-type) | Compound | Cav2.2b (N-type) |
|---|---|---|---|
| EtOH extract | 36.3 ± 10.2% | 1 | 43.8 ± 1.7% |
| EtOAc-part | 48.6 ± 7.7% | 2 | 3.2 ± 2.1% |
| n-BuOH-part | – | 3 | 38.9 ± 6.7% |
| H2O-part | 1.1 ± 1.7% | 4 | 4.1 ± 2.4% |
| Fr.1 | 1.3 ± 6.3% | 5 | 9.0 ± 16.2% |
| Fr.2 | 56.5 ± 4.8% | 6 | 8.5 ± 7.0% |
| Fr.3 | 10.5 ± 6.2% | 7 | 0.1 ± 1.1% |
| Fr.4 | 68.6 ± 3.1% | 8 | 3.4 ± 1.9% |
| Fr.5 | 55.2 ± 3.5% | 9 | 5.4 ± 1.5% |
| Fr.6 | 60.1 ± 4.0% | ||
| Fr.7 | 34.1 ± 6.3% | ||
| Fr.8 | 28.4 ± 5.2% | ||
| Fr.9 | 14.1 ± 2.0% |
aPeak current inhibition ratio of extracts at 0.01 mg/ml; bPeak current inhibition ratio of compounds at 10 μM (1and3) or 30 μM (other compounds); Data represent the mean ± SEM of 3 or 4 cells.