Fig.2.

Differentiation of hTSCs into hOLCs in specific culture medium. A-D. Round cells 20-25 µm in diameter were observed at day 25 to approximately day 40 of differentiation, E, F. hOLCs could be further developed when cultured in OLC medium and co-cultured with human granulosa cells, and G-I. Differentiation of hTSCs into follicle-like structures. Cell aggregates, which resemble follicle-like structures, appeared at days 25-30 of differentiation cells (scale bars: 10 µm).

hOLCs; Human oocyte like cells and hTSCs; Human theca stem cells.