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. 2018 Aug 7;20(4):537–543. doi: 10.22074/cellj.2019.5639

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Fig.1 — FloCytogram from analysis of 106 spermatozoa by sperm chromatin structure assay (SCSA) and caspase activity of spermatozoa with FAM- DEVD-FMK/propidium iodide (PI). A. Density plot of sperm cells by SCSA FL1: Green fluorescent double-strand DNA (dsDNA); FL3: Red fluorescent single-strand (ssDNA); R1: Number of spermatozoa with DNA fragmentation; R2: Number of spermatozoa with dsDNA. Debris (bottom left corner) were excluded from the analysis and B. Density plot of sperm cells stained with FAM-DEVD-FMK/PI. Density plot shows viable, unstained spermatozoa in lower left quadrant (negative for FAM-DEVD-FMK and PI); live spermatozoa with caspase activity in lower right quadrant (FAM- DEVD-FMK positive only); and dead spermatozoa in upper right quadrant (positive for FAM-DEVD-FMK and PI) .