Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 7;20(4):576–583. doi: 10.22074/cellj.2019.5589

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig.5 — Immunofluorescence staining for HbF production and Real time evaluation for BCL11a, HDAC and Gamma-globin genes expression. A. Results of immunofluorescence staining showed that ROM and SIM can induce HbF production in erythroid progenitors differentiated form cord hematopoietic stem cells: 1) Newborn blood was used as positive control for immunofluorescence staining, 2) Untreated group, 3) ROM group, 4) SIM group, 5) ROM/SIM group (×100) and B. CD34+ cells were treated with ROM, SIM and ROM/SIM for 7 and 14 days. Results of gene expression showed that ROM and SIM can induce Gamma-globin gene expression and downregulate BCL11a and HDAC1 genes expression on the 7^th and 14^th day, but the combination of ROM and SIM can induce Gamma-globin gene transcription synergistically by downregulation of BCL11a and HDAC1 genes on the 7^th and 14^th day. We also found that ROM and SIM had no effect on HDAC2 gene expression.

*; P<0.05, HbF; Fetal hemoglobin, ROM; Romidepsin, SIM; Simvastatin, and SB; Sodium butyrate.