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. 2018 Apr 16;155(1):85–98. doi: 10.1111/imm.12926

Figure 2.

Figure 2

Arg I and Arg II expressions were differentially regulated during dendritic cell (DC) differentiation and activation. In (a) and (b), DCs were generated from C57BL/6J mouse bone marrow cells in the presence of IL‐4 and granulocyte macrophage‐colony‐stimulating factor (GM‐CSF) 27 (Materials and methods). On day 5, DCs were purified with CD11c‐MACS beads, seeded in 12‐well plates and treated with vehicle (cntr), lipopolysaccharide (LPS; 0·5 μg/ml), CD40L (1 μg/ml), TLR8 agonist R848 (1 μg/ml), IL‐4 (20 ng/ml) or GM‐CSF (50 ng/ml) for 36 hr. The mRNA level of Arg I (a) and Arg II (b) was determined by quantitative polymerase chain reaction (qPCR) and adjusted for GAPDH expression. In (c) and (d), purified DCs were treated with either vehicle (cntr) or LPS (0·5 μg/ml) or IL‐4 (20 ng/ml) for 36 hr. Data represent the means ± SEM (n = 4). *P < 0·05, **P < 0·01, ***P < 0·001: versus cntr.