Figure 7.

Arg II deficiency significantly reduced the abundance of Th17 cells and IL‐23⁺ cells in the draining lymph nodes (LNs). Wild‐type (WT) and Arg II knockout (KO) mice (n = 5) were subjected to myelin oligodendrocyte glycoprotein peptide (MOG) immunization (same experiment as described in Fig. 6). Mononucleated cells isolated from draining LNs of WT experimental autoimmune encephalomyelitis (EAE) mice and Arg II KO EAE mice were subjected to various cell surface marker or intracellular cytokine staining as indicated. (a). Representative Th17 FACS histograms were shown here. (b) Statistical analysis of the gated viable and CD3⁺/CD4⁺ IL‐17⁺ lymphocytes (three WT EAE mice and three Arg II KO mice). Mean ± SEM, *P < 0·05 (WT EAE versus Arg II KO EAE). (c and d) Lymphocytes that were pooled from two WT EAE mice or two Arg II KO mice were subjected to FACS analysis of IL‐23p19 (c) or IL‐23/IL‐12p40 (d).