Figure 3.

Hydrogen peroxide (H₂O₂) secretion is altered by alphaB‐crystallin (α BC) treatment of stimulated neutrophils. Hydrogen peroxide, as an indicator of reactive oxygen species (ROS), secreted by zymosan‐stimulated neutrophils following (a) 2 hr, (b) 4 hr and (c) 20 hr pre‐stimulation with granulocyte macrophage‐colony‐stimulating factor (GM‐CSF) + lipopolysaccharide (LPS) with (squares) or without (circles) addition of α BC. The results represent the combined mean and spread of five (2 hr), five (4 hr) and five (20 hr) independent experiments. Statistical analyses were performed by repeated‐measures two‐way anova with Šídák post hoc test (2 and 4 hr), and repeated‐measures one‐way anova with Dunnett's multiple comparison test for 20 hr, *P < 0·05 of main effect (2 hr), interaction (4 hr stimulation) and post hoc/multiple comparison tests (4 and 20 hr treatment).