Skip to main content
. 2018 Apr 17;155(1):72–84. doi: 10.1111/imm.12924

Figure 4.

Figure 4

Stimulated neutrophils previously treated with alphaB‐crystallin (α BC) induced less interleukin (IL)‐12p40 production by DCs. (a) Flow cytometric gating strategy to determine purity of bone marrow‐derived DCs after culturing for 1 week in granulocyte macrophage‐colony‐stimulating factor (GM‐CSF)‐containing media, and 48 hr in GM‐CSF‐free media. The purity of DCs from three separate experiments is shown. (b–g) The presence of IL‐1β (b–d) and IL‐12p40 (e–g) in the supernatants of DCs co‐cultured with neutrophils previously stimulated with GM‐CSF + lipopolysaccharide (LPS) with (squares) or without (circles) of α BC in a 1 : 5 (b, e), 1 : 2·5 (c, f) or 1 : 1 (d, g) DC : neutrophil ratio. The data represent the mean and spread of data from (b) six, (c) three, (d) three, (e) six, (f) three and (g) four individual experiments. Statistical analyses were performed by two‐way anova with Šídák post hoc test, *P < 0·05 of main effect (stimulation on IL‐1β and IL‐12p40) and post hoc test (treatment on IL‐12p40).