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. 2018 Aug 13;9:1865. doi: 10.3389/fimmu.2018.01865

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Copyright © 2018 Dufour, Alvarez, Russo and Chizzolini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-6 and monocyte chemotactic protein-1 (MCP-1) are synergistically and specifically induced in human fibroblasts by the combined action of IL-17A and TGF-β. Primary human dermal fibroblasts from healthy donors (HD) and systemic sclerosis (SSc) patients were cultured in the presence of IL-17A (25 ng/ml), TGF-β (2.5 ng/ml), or their combination for 48 h, in 96-well plates, in triplicates. IL-6 (A), MCP-1 (B), IL-8 (C) were assessed by ELISA in culture supernatants. Results are expressed as fold change compared to spontaneous production in control (ctrl) cultures. Basal levels were: 22.7 (±7.3) and 40.7 (±16.7) pg/ml for IL-6; 328.1 (±33.3) and 377.2 (±85.4) pg/ml for MCP-1 and 211.8 (±56.6) and 207.7 (±56.9) pg/ml for IL-8, in HD and SSc, respectively. Significance was assessed by paired t test.