Figure 2.

APRIL transcription levels in early rainbow trout developmental stages. Transcriptional levels of APRIL during trout early development at different stages were estimated by real-time PCR. Data are shown as the gene expression relative to the expression of an endogenous control (EF-1α) (mean + SD, n = 5). Abbreviations: DD, degree days; HAT, hatching; PFF, 1 week before first feeding; FF, first feeding; Fry, 3 weeks post-first feeding. Asterisks indicate levels of expression significantly different to those observed in samples taken at 306 DD (**p ≤ 0.01 and ***p ≤ 0.005).