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. 2018 Aug 13;9:1867. doi: 10.3389/fimmu.2018.01867

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Copyright © 2018 Conroy, Maher, Melo, Doyle, Foley, Reynolds, Long and Lysaght.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CX3CR1^NEG and CX3CR1^INT populations represent significantly different CD8⁺ T cells in the peripheral blood with significantly different memory phenotypes, L-selectin, and ICAM-1 expression. (A) Bar charts showing the frequencies of naive, central memory, effector memory, and terminally differentiated CD8⁺ T cells, characterized by CD45RA and CD27 expression within the CX3CR1^NEG and CX3CR1^INT populations in peripheral blood of six non-cancer controls. (B) Bar chart showing frequencies of peripheral blood-derived CD8⁺ T cells expressing CD45RA (top) and CD27 (bottom) within CX3CR1^NEG and CX3CR1^INT populations (n = 3). (C) Bar chart showing frequencies of peripheral blood-derived CD8⁺ T cells ICAM-1 and L-selectin within CX3CR1^NEG and CX3CR1^INT populations (n = 3). p < 0.05, **p < 0.01, ***p < 0.001 by unpaired t tests.