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. 2018 Aug 13;9:1805. doi: 10.3389/fimmu.2018.01805

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Copyright © 2018 Lauzon-Joset, Jones, Mincham, Thomas, Rosenthal, Bosco, Holt and Strickland.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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vMC₀-induced airways inflammation in PVG and BN. Bronchoalveolar lavage (BAL) was obtained from naïve PVG () and BN () at one and 3 days post-vMC₀ infection (dpi). (A) BAL total cell count, as well as (B) neutrophils, (C) macrophages, and (D) lymphocytes were enumerated using trypan blue and Diff-Quick staining. (E) Viral load was assessed in BAL of PVG and BN by plaque assay (n = 3–6). Statistical analysis was carried out by non-parametric Kruskal–Wallis test followed by Dunn’s multiple comparisons [overall p-values for panels (A–E) are respectively: 0.0002; 0.0095; 0.0001; 0.0856; and 0.0001].

Inline graphic — vMC₀-induced airways inflammation in PVG and BN. Bronchoalveolar lavage (BAL) was obtained from naïve PVG () and BN () at one and 3 days post-vMC₀ infection (dpi). (A) BAL total cell count, as well as (B) neutrophils, (C) macrophages, and (D) lymphocytes were enumerated using trypan blue and Diff-Quick staining. (E) Viral load was assessed in BAL of PVG and BN by plaque assay (n = 3–6). Statistical analysis was carried out by non-parametric Kruskal–Wallis test followed by Dunn’s multiple comparisons [overall p-values for panels (A–E) are respectively: 0.0002; 0.0095; 0.0001; 0.0856; and 0.0001].