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. 2018 Jun 25;62(14):1701043. doi: 10.1002/mnfr.201701043

Figure 6.

Figure 6

Effect of l‐citrulline on the gene expression of PGC‐1α in C2C12 myotubes. Differentiated C2C12 myotubes were treated with or without l‐citrulline for 24 h. A) After that, cell viability was evaluated and value expressed as percentage (%) of control. B) C2C12 myotubes were treated with or without l‐citrulline (10, 50, 100 μM) for 1 h. C) l‐citrulline (50 μM) treatment was performed with or without 100 μM NG‐nitro‐l‐arginine methyl ester hydrochloride (l‐NAME) for 1 h. Following treatment, PGC‐1α mRNA levels were quantified using real‐time PCR and the values normalized to the expression level of β‐actin. Values are expressed as the mean ± standard deviation of triplicate experiments. **p ≤ 0.01 indicates a significant difference from the control group. ## p ≤ 0.01 indicate a significant difference from the l‐NAME‐treated l‐citrulline group.