Table 3.

Primers used for knockdown, knockout and rescue constructs. Upper case letters refer to gene sequences and enzyme restriction sites are underlined

Primer name	Sequence
ORF‐BamHI forward	5′‐ggatccaATGGCGGACATCTTGAAG‐3′
ORF‐BamHI reverse	5′‐ggatccTCAAGATGAAACTCTCGA‐3′
5′UTR‐NotI forward	5′‐ataagaatgcggccgcCGCTAATGATTACACTAC‐3′
5′UTR‐HindIII/PmeI reverse	5′‐gtttaaacttacggaccgtcaagcttGCTTGATTCCGCGTGGAC‐3′
3′UTR‐PmeI/BamHI forward	5′‐gacggtccgtaagtttaaacggatccAGCGCTTAGGGATGTCAC‐3′
3′UTR‐NotI reverse	5′‐ataagtaagcggccgcGTTCGATACCGTTTTGAGA‐3′
G5K‐BamHI forward	5′‐gcgcggatccATGGCGGACATCTTGAAGT‐3′
G5K‐EcoRI forward	5′‐gcgcgaattcTCAAGATGAAACTCTCGAAT‐3′