Figure 8.

Adenoviral over‐expression of PGC‐1α reduced Drp1 expression and inhibited mitochondrial fission. (A) Representative confocal microscope images showing mitochondrial morphology stained by MitoTracker Red (major finding is D). Original magnification ×600. (B,E,F) Protein expressions of PGC‐1α and Drp1 were determined by Western blotting. (C) ChIP analysis for PGC‐1α binding to the Drp1 promoter in H9c2 cells. (D) The percentage of cells with fragmented mitochondria. Presented values are means ± SEM. NG, normal glucose (5.5 mmol/L); HG, high glucose (33 mmol/L glucose). n = 6 in each group. *P < .05, **P < .01 vs NG with Ad GFP. ## P < .01 vs HG with Ad GFP