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. 2018 Jun 25;70(9):1228–1241. doi: 10.1111/jphp.12950

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© 2018 The Authors. Journal of Pharmacy and Pharmacology published by John Wiley & Sons Ltd on behalf of Royal Pharmaceutical Society

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Effect of Salvia miltiorrhiza Bunge treatment on expression level of apoptosis‐related proteins in mesenchymal stem cells (a) Following treatment of S. miltiorrhiza Bunge and normoxic and hypoxic conditions, western blotting using antibodies Bcl‐2, Caspase‐3 and Bax was performed for detecting apoptosis‐related factors. (b) After treatment of S. miltiorrhiza Bunge in each condition, relative folds of Bcl‐2 (anti‐apoptosis related factor), Caspase‐3 and Bax (pro‐apoptosis related factors) were calculated by normalization to β‐actin. The expression level of pro‐apoptosis related factors in Hypoxia‐S. miltiorrhiza Bunge group was 6.5‐ and 7.6‐fold lower than in Hypoxia‐control group. On the other hand, the expression level of anti‐apoptosis‐related factor in Hypoxia‐S. miltiorrhiza Bunge group was 2.0‐fold higher than in Hypoxia‐control group. Columns, mean; bars, SE. *P < 0.05.