Figure 7.

Inhibition IgE immunoblot with rPor p 1. Patient serum was preincubated with rPor p 1 at 0.4 μg mL⁻¹ (iii), 2 μg mL⁻¹ (iv), and 10 μg mL⁻¹, (v) and immunoblotted against: cooked calamari extract A) patient A5, B) patient A9; cooked mussel extract C) patient A9, D) patient A5; cooked scallop extract E) patient A9, F) patient A13; and cooked oyster extract G) patient A12, H) patient A10. No inhibitor (i) and OVA at 20 μg mL⁻¹ (vi) were used as negative controls. Same inhibitor as extract was used as a positive control (ii).