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Pex1p, Pex2p and Pex13p play a role in targeting HpAat2p to peroxisomes (A) Fluorescence microscopy images of WT, pex1.atg1, pex2 and pex13 cells grown for 16 h on ethanol containing media. Besides the peroxisomal marker Pex14‐mCherry, all cells produced the fusion protein Aat2‐GFP. Scale bar represents 1 μm. (B) SDS/PAGE and immunoblot analysis of lysates from the deletion strains presented in (A) probed with antibodies directed against GFP or Pyc‐1 (loading control).
