Figure 5.

6‐Dihydroparadol increases A) ABCA1, but not B) ABCG1 mRNA levels in cholesterol‐loaded THP‐1‐derived macrophages. THP‐1 cells were differentiated as described in Figure 2, and then loaded with unlabeled cholesterol for further 24 h. Cells were treated with 6‐dihydroparadol (5 and 10 μm) for another 24 h. The control was treated with solvent vehicle (0.1% DMSO) for 24 h. As a positive control, the LXR agonist TO901317 (5 μm) was used. The mRNA levels of ABCA1 and ABCG1 were detected by RT‐qPCR. Bar graphs represent mean ± SD from three independent experiments. *p < 0.05 and ***p < 0.001 versus control (determined by Student's t‐test or ANOVA with Bonferroni's post hoc test).