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. 2018 Aug;188(8):1779–1793. doi: 10.1016/j.ajpath.2018.05.005

Figure 5.

Figure 5

Lysophosphatidic acid (LPA) precursor expression precedes expression of neurodegeneration in the thalamus after injury. A: Mean ipsilateral thalamic regions of interest (ROIs; red circle) pixel intensity measurements of intracellular LPA precursors were acquired from representative matrix-assisted laser desorption ionization (MALDI) sections at bregma –1.8 mm from all three groups. B: Normalized plot of thalamic ROI data at 1 and 3 hours after injury (HPI). C: Brain atlas at bregma –1.8 mm showing the region where representative images were taken of necrotic/injured Fluoro-Jade B (FJ-B)–positive cells (examples shown with arrowheads) in sections adjacent to the MALDI images in uninjured controls, 1 hour after injury, and 3 hours after injury. D: Plot of total FJ-B–positive cell counts in thalamic regions (two rats/group) and the significant postinjury increase in number of degenerating cells. Data are expressed as means ± SEM (B). n = 3 per group (B). ∗P < 0.05, ∗∗∗P < 0.001 (two-tailed t-test, 95% CI in R). Scale bar = 100 μm (C). LPC, lysophosphatidylcholine; PA, phosphatidic acid; PE, phosphatidylethanolamine.