Table 1.
Phospholipid Species Detected in Specified Regions of Interest
| Phospholipid class | m/z | Adduct | + Ion | Cortex |
Pericontusion cortex |
White matter |
Sagittal (brain wide) |
|||
|---|---|---|---|---|---|---|---|---|---|---|
| 1 HPI | 3 HPI | 1 HPI | 3 HPI | 1 HPI | 3 HPI | 3 HPI | ||||
| Heme | 616.16 | NA | NA | *** | *** | NA | NA | *** | *** | Increase |
| LPA | 477.222 | 18:0 | K+ | — | — | * | ** | — | — | Decrease |
| LPA | 459.2482 | 18:1 | Na+ | — | * | *** | *** | — | — | Increase |
| LPA | 590.3426 | 20:0 | K+ | — | — | * | * | — | — | ND |
| LPA | 503.29 | 20:1 | K+ | — | ** | * | — | — | — | Decrease |
| PA | 713.443 | 16:0/18:1 | K+ | * | — | — | — | * | — | Increase |
| PA | 739.46 | 18:0/18:2 | K+ | ** | ** | — | — | *** | *** | Increase |
| PA | 709.49 | 34:3 | K+ | — | — | — | — | — | — | Increase |
| LPC | 562.32 | 18:0 | K+ | *** | * | — | — | * | * | Increase |
| LPC | 560.31 | 18:1 | K+ | ND | ND | ND | ND | ND | ND | Increase |
| LPC | 540.26 | 18:3 | Na+ | *** | *** | — | — | *** | *** | Increase |
| LPC | 588.52 | 20:1 | K+ | — | ** | — | — | * | — | ND |
| LPC | 630.448 | 24:0 | Na+ | — | — | * | * | — | — | Increase |
| PC | 798.5 | 34:1 | K+ | ** | ** | * | * | ** | ** | Increase |
| LPE | 54,634 | 20:1 | K+ | — | — | * | — | — | — | ND |
| LPE | 524.26 | 20:4 | Na+ | — | — | * | * | — | — | Increase |
| PE | 518.26 | 18:1 | K+ | — | * | — | — | * | * | Increase |
| DAG | 607.5574 | 32:0 | K+ | — | — | * | — | — | — | Increase |
| S1P | 379.3 | NA | NA | — | — | * | * | — | — | Decrease |
Adducts represented as carbon chain length: number of double bonds; spectra acquired in positive ion mode, potassium (K+) and sodium (Na+). For each region of interest (ipsilateral cortex, pericontusion cortex, and ipsilateral white matter), n = 3 per group per time period; P values for pixel intensities increase (green) or decrease (red) from control value pixels and were determined by bootstrapping the 95% CIs around the median and two-way analysis of variance for each time period and each region of interest in R. Spectra not detected for a given lipid indicated the phospholipid species that were not gathered in the mass spectrometry run. Sagittal brain-wide changes are indicated by pixel intensity differences between sagittal region of interest of control and sagittal region of interest of 3 HPI (n = 1 per group). Brain-wide pixel intensities at 3HPI are indicated as increase or decrease from control pixel intensities.
∗P ≤ 0.05, ∗∗P ≤ 0.01, and ∗∗∗P ≤ 0.001.
–, Pixel intensities for a given phospholipid that did not change from control value (P > 0.05); DAG, diacylglycerol; heme, phospholipid class, hemeoglobin; HPI, hours after injury; LPA, lysophosphatidic acid; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; m/z, mass/charge ratio; NA, not applicable; ND, not detected; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; S1P, sphingosine-1-phosphate.