Figure 2.
Tumor-specific expression of glypican3 in hepatocellular carcinoma. (A) Quantification of Gpc3 mRNA transcripts within tumors (T) and surrounding liver tissue (S) in wildtype (Klrk1+/+) and NKG2D-deficient (Klrk1−/−) diethylnitrosamine (DEN)-treated mice (n ≥ 17) and AMC (n = 9). (B) Representative IHC staining of GPC3 on a DEN-treated liver where T represents the tumor area and S represents the surrounding tissue. Graph represents the mean ± SEM. Statistical analysis was performed by unpaired Student’s t test, where statistically significant differences between groups are denoted as: **p ≤ 0.01 and ***p ≤ 0.001.