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. 2018 Jun 20;57(33):10559–10563. doi: 10.1002/anie.201804662

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© 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Representative hyperspectral TPEFM images (λ _ex=720 nm) of GUVs composed of a) L_d DOPC, b) L_o SM/CL 7:3, and c) SM/DOPC/CL 58:25:17 (two different GUVs) in the presence of papillon 2 (color bar: emission wavelengths in the 400–650 nm range), with normalized emission spectra of entire GUVs composed of d) DOPC and e) SM/CL 7:3 with 0.5 μm (red), 1.0 μm (green), and 2.0 μm (blue) 2, and f) spectrally unmixed images of GUVs in (c) using reference spectra in (d) and (e) showing L_d (red) and L_o domains (green). Scale bar: 20 μm.