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Tricine-SDS-PAGE analysis of supernatant with 1.5 mM IPTG induction. Lane 1, supernatant of non-induced E. coli BL21 (DE3); Lane 2, 3, 4, 5, 6 were supernatant of 1.5 mM IPTG-induced E. coli BL21 (DE3) for 1, 2, 3, 4, 5 h respectively; Lane M, marker. The arrows indicated fusion protein SUMO–C–L.
