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Tricine-SDS-PAGE analysis of purified fusion protein SUMO–C–L. Lane M, the molecular weight of marker; Lane 1, the supernatant of IPTG-induced E. coli BL21 (DE3); Lane 2, the supernatant of non-induced bacterial lysate; Lane 3, the purified fusion protein SUMO–C–L, arrow in the lane indicated the fusion protein SUMO–C–L; Lane 4, the enzymatic reaction solution of fusion protein, arrows in the lane were SUMO and recombinant C–L.
