Transcription of fatty acid biosynthesis genes is typically unaffected by the loss of staphyloxanthin production in S. aureus. (a) Fold change in gene expression in Pig1ΔcrtM compared to that in Pig1 grown in TSB; (b) Fold change in gene expression in Pig1ΔcrtM compared to that in Pig1 grown in MHB; (c) FapR recognition sequence (operator sequence) in the gene or operon involved in fatty acid biosynthesis and regulation in S. aureus NCTC 8325. The transcriptional level of each gene was normalized to that of 16SrRNA. The dashed line in (a,b) indicates similar level of gene expression in the Pig1 pair strains. fabZ operon, SAOUHSC_02337–02336; plsC, SAOUHSC_01837; fapR operon, SAOUHSC_01196–01199; fakA operon, SAOUHSC_01192–01193; accA operon, SAOUHSC_01809–01808; fabH operon, SAOUHSC_00920–00921; fabI, SAOUHSC_00947; lpd operon, SAOUHSC_01617–SAOUHSC_01615–01611; BCAT, SAOUHSC_00536–00537. The number in parentheses after each operator sequence in (c) indicates the last nucleotide in the given sequence. # [30].