Figure 8.

SSA inhibition using site-directed anti-peptide antibodies against Tf. (a) Localization of antigen epitopes for site-directed anti-peptide antibodies on a 3D-model of Tf. One antibody, Tf-596Ab, was prepared against a peptide sequence, Cys596-Ala614, (highlighted with red). The other antibody, Tf-120Ab, was prepared against a peptide sequence, Val120-Cys137, (highlighted with green). Asn-432 and Asn-630 are N-glycosylation sites. (b) A pair of mirror image sections is stained with Tf-596Ab (upper panel) or Tf-120Ab (lower panel) in the presence or absence of SSA. Interlobular and central veins are indicated with red and blue arrowhead, respectively. The data is reproduced with permission from Matsumoto et al., J. Biochem., Oxford University Press, 2014 [11].