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Effects of APTH and compound 10 on NF-κB signaling pathways in LPS-treated RAW264.7 macrophages. Cells were pretreated for 1 h in the presence or absence of samples and then stimulated for 1.5 h with LPS (0.1 μg/mL). Cell cytosolic and nuclear extracts were prepared to determine whether IκB phosphorylation levels and p65 translocation to the nucleus. β-Actin and Lamin B were used as loading controls. Dexamethasone served as the positive control.
