Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Analytical conditions of high-performance liquid chromatography (HPLC) system to analyze the three markers.
| Parameters | Conditions | ||
|---|---|---|---|
| Column | Zorbax extended-C18 (C18, 4.6 mm × 150 mm, 5 µm) |
||
| Flow rate | 0.8 mL/min | ||
| Injection volumn | 10 μL | ||
| UV detection | 230 nm | ||
| Run time | 35 min | ||
| Gradient | Time (min) | A(%) | B(%) |
| 0 | 10 | 90 | |
| 10 | 10 | 90 | |
| 20 | 20 | 80 | |
| 25 | 30 | 70 | |
| 27 | 100 | 0 | |
| 28 | 10 | 90 | |
| 35 | 10 | 90 | |
