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. 2018 Jun 27;23(7):1557. doi: 10.3390/molecules23071557

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Modulations of intracellular signaling pathways. Cells were treated with samples, or control for 72 h. Total protein was extracted for the analysis. A slide-based antibody array was used for simultaneous detection of 18 signaling molecules (a) when phosphorylated or cleaved using a PathScan Intracellular Signaling Array kit. Image (b) and quantitative data (c) for MCF-7 cells as well as image (d) and quantitative (e) data for MDA-MB-23 cells were obtained using a LiCor Odyssey imaging system. Data are presented as mean ± SD. * indicates a significant difference compared to the control (p < 0.05). # indicates a significant difference compared to treatment with samples by single (p < 0.05).

Modulations of intracellular signaling pathways. Cells were treated with samples, or control for 72 h. Total protein was extracted for the analysis. A slide-based antibody array was used for simultaneous detection of 18 signaling molecules (a) when phosphorylated or cleaved using a PathScan Intracellular Signaling Array kit. Image (b) and quantitative data (c) for MCF-7 cells as well as image (d) and quantitative (e) data for MDA-MB-23 cells were obtained using a LiCor Odyssey imaging system. Data are presented as mean ± SD. * indicates a significant difference compared to the control (p < 0.05). # indicates a significant difference compared to treatment with samples by single (p < 0.05).