Figure 5.

Membrane binding and leakage activity of Gomesin variants. (A) Binding isotherms showing molar fraction of bound peptide (Xb) as a function of free peptide concentration (C_eq) obtained from ITC experiments of Gm and its variants binding to LUVs composed of POPC–POPG 7:3 (mol ratio); (B) Top panel: Kinetics of CF leakage from LUVs composed of POPC–POPG 7:3 (mol ratio) after addition of Gm and its variants at a peptide-molar ratio of 0.12 at room temperature (~23 °C). Bottom panel: percentage leakage as a function of peptide-lipid molar ratio; (A,B) were adapted with permission from Mattei et al. [28]. Copyright 2014 American Chemical Society; (C). Binding of cyclic Gm (cGm) and its variants to model membranes composed of POPC and POPC–POPG (4:1 molar ratio) from surface plasmon resonance (SPR) experiments. Left panel: sensorgrams from SPR experiments using a peptide concentration of 32 μM. Right panel: Dose-response binding curves from the different peptide-lipid concentrations. Response units were converted into moles of peptide and normalised for the amount of peptide. Adapted with permission from Henriques et al. [9]. Copyright 2017 American Chemical Society.