Table 3.

Thermodynamic parameters determined by isothermal titration calorimetry (ITC) measurements of the binding of Gomesin to membranes composed of POPC with different fractions of POPG. Data from binding isotherms was fitted using a Langmuir model or a partition model.

Membrane Composition (Mol Ratio)	Binding Model	KA (μM−1)	KD (1/KA) (μM)	ΔG (kJ mol−1)	ΔH (kJ mol−1)	TΔS (kJ mol−1)
POPC–POPG (1:1) [25]	Langmuir	5	0.2	−27.6	−33.4	−5.8
POPC–POPG (1:1) [25]	Partition	0.01	100	−12.6	−32.6	−20.0
POPC–POPG (1:1) [26]	Partition	0.5	2	−22.1	−29.2	−7.1
POPC–POPG (3:1) [26]	Partition	0.01	100	−12.6	−23.4	−10.8
POPC–POPG (7:3) [28]	Partition	125 × 10−3	8.0 × 103	−2.0	−33.0	−31.0