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. 2018 Jul 30;115(33):E7738–E7747. doi: 10.1073/pnas.1808146115

Fig. 5.

Fig. 5.

RNAi-mediated knockdown of AaTai-A and AaTai-B impedes ovarian development and ecdysteroid response. DsRNAs for the AaTai common region (dsTaiCore), AaTai-E12 (dsTaiE12), and AaTai-E13 (dsTaiE13) were injected into adult female mosquitoes within 30 min PE. DsGFP was used as a control. (A and B) Ovaries isolated from each group of mosquitoes at 96 h PE and 24 h PBM. Non-inj, noninjected. (Scale bars: 500 µm.) (C and D) Average lengths of primary follicles were measured at 96 h PE and 24 h PBM. Thirty individuals were measured for each group, and the results are presented as the mean ± SD. (E) Expression of the JH response genes AaKr-h1 and AAEL002576 in the fat body at 96 h PE. qRT-PCR results are presented as a fold change compared with the dsGFP-injected sample. Results are the mean ± SD of three independent experiments. (F) Expression of AaE74B, AaE75A, and AaVg at 24 h PBM. qRT-PCR results are the mean ± SD of three independent experiments. Statistical significance between the groups is shown (*P < 0.05; **P < 0.01; ***P < 0.001).