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. 2018 Jul 30;115(33):E7738–E7747. doi: 10.1073/pnas.1808146115

Fig. 7.

Fig. 7.

Binding of individual AaTai isoforms to the AaEcR–AaUSP complex and AaMet. (A) Protein interaction between AaTai and AaEcR/AaUSP. Drosophila L57 cells were transfected with the expression plasmids for the V5-tagged AaTai isoforms, together with the expression vectors for AaEcR and AaUSP. The transfected cells were treated with 20E (1 μM) or ethanol. AaEcR in the cell extracts was precipitated by anti-AaEcR antibody, and the pellets were analyzed by immunoblotting using anti-V5 antibody. Input [10% of the amount of cell lysate used for the immunoprecipitation (IP)] was analyzed using anti-V5, anti-AaEcR, anti-AaUSP, and anti-GAPDH antibodies. (B) Coimmunoprecipitation of AaTai isoforms with AaMet. Drosophila L57 cells were transfected with the expression plasmids for the V5-tagged AaTai isoforms and for AaMet. The transfected cells were treated with JH-III (1 μM) or ethanol. Immunoprecipitation was performed using an anti-AaMet antibody, and Western blotting was performed using anti-V5 antibody. Input was analyzed using anti-V5, anti-AaMet, and anti-GAPDH antibodies.