Fig. 4.

DDX11 acts jointly with 9-1-1 in postreplicative DNA repair. (A) DNA replication speed in the presence of DNA damage. The ratio of IdU/CldU track was plotted. For each sample, more than 70 fibers were measured (WT untreated, n = 102; WT MMS n = 78; ddx11 untreated, n = 90; ddx11 MMS, n = 85). In the box plots, the middle line indicates the median value; the box shows the 25th and 75th percentiles; the bars, the 5th and 95th percentiles. (B) Sensitivity of cells as in Fig. 1B. Note overlap between rad17 and ddx11 rad17 curves upon CDDP treatment. (C) Chk1 phosphorylation after CDDP treatment. Histone H3 was used as a loading control. (D and E) Sensitivity of cells of the indicated genotypes as in Fig. 1B.