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ChIP assay demonstrating repression of HIV-1 transcription by ESR-1. Latently infected Th17 cells were activated through the TCR by CD3/CD28-coated magnetic beads for either 0.5 or 16 h in the presence or absence of 1 μM ICI or 300 pg/mL β-estradiol. (A) Location of primers used to detect protein interactions at the 5′ and 3′ LTRs of the HIV-1 provirus. (B) RNAP II pSer5. (C) RNAP II pSer 2. (D) ESR-1. (E) β-catenin.
