Fig. 5.

EDITS estimate of the inducible RNA reservoir size in females and males. EDITS assays were performed on unstimulated cells or cells activated by TCR stimulation (maximal activation) in the presence and absence of 300 pg/mL β-estradiol, 1 μM ICI, 500 nM SAHA, or a combination of ICI and SAHA. For each donor, the EDITS assays were performed on three separate occasions using separate aliquots (biological replicates). Libraries were prepared from the isolated RNAs and were sequenced in two separate runs (technical replicates). Solid circles: PGM sequencer. Open symbols: S5 sequencer. Males respond statistically better than females to TCR stimulation (P value = 0.005), indicating a larger inducible reservoir size.