Skip to main content
. 2018 Jul 31;115(33):E7758–E7767. doi: 10.1073/pnas.1805713115

Fig. 2.

Fig. 2.

Humoral immune responses induced in immunized mice. (A) Immunization and challenge experiment timeline. Immunization groups include soluble peptide mixture of 4M2e and the NP peptide (4M+NPep), NP-peptide/4M2e–layered nanoparticles (NPep/4M2e nano), NP-protein/4M2e–layered nanoparticles (NP/4M2e nano), OVA nanoparticles (OVA nano) and DPBS. (B) M2e-specific IgG endpoint titers. IgG titers against 4M2e were determined with serum samples collected 3-wk postboosting. (C) Determination of IgG1 and IgG2a titers against 4M2e using ELISA. (D) Bar chart showing logarithm values of the ratio IgG1:IgG2a. (E) M2e binding spectrum. Serum IgG binding capacity to various M2e peptides, including p09M2e, VtnM2e, and SHM2e was evaluated. Data are presented as mean ± SD (n = 10). Statistical significance was analyzed by t test for B, C, and E. P values shown in bar charts and N.S. indicates no significance between two compared groups. The experiments were repeated twice with the same samples with similar results.