Fig. 3.

Cellular immunity. (A) Cellular immune response specific to M2e, NP₁₄₇, and NP₅₅. Peptide pool-stimulated IFN-γ–secreting cell clones were determined using ELISpot assay (n = 4). (B–D) Splenocyte cytokine secretion. Lymphocytes from immunized mouse spleens were stimulated with a peptide mixture containing 4M2e and NP peptides. The levels of secreted cytokines including IFN-γ (B), IL-4 (C), and IL-2 (D) in the splenocyte culture medium were tested with cytokine ELISA. Data are presented as mean ± SD (n = 3). Statistical significance was analyzed by t test. P values shown in bar charts and N.S. indicates no significance between two compared groups. The experiments were repeated twice with the same samples with similar results.