Fig. 1.

StNRL1 interacts with itself and StSWAP70 in yeast and in planta and localizes to vesicles. (A) Yeast coexpressing StNRL1 with itself or StSWAP70 grew on −HIS medium and yielded β-Gal activity, while those coexpressed with the control EV did not. The +HIS control shows all yeast were able to grow in the presence of histidine. (B and C) The interactions were further confirmed by co-IP assay using expression in N. benthamiana leaves. cMYC-StNRL1 associated with GFP-StSWAP70 and GFP-StNRL1, whereas cMYC-GUS did not. Expression of constructs in the leaves is indicated by a “+.” Protein size markers are indicated in kilodaltons, and protein loading is indicated by Ponceau stain (PS). (D) Confocal images showing that GFP-StSWAP70 is localized in the cytoplasm and in vesicles. (Scale bars, 10 μM.) The Inset is an enlarged image of the nucleus.