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. 2018 Jul 26;115(33):E7834–E7843. doi: 10.1073/pnas.1808585115

Fig. 3.

Fig. 3.

Pi02860 promotes proteasome-mediated degradation of StSWAP70 in an NRL1-dependent manner. (A) Coexpression of GFP-StSWAP70 with cMYC-StNRL1 and/or cMYC-Pi02860 causes reduced StSWAP70 abundance, which was prevented by MG132 treatment. (B) GFP-StSWAP70 was transiently expressed alone or with cMYC-Pi02860 in TRV:GFP and silenced TRV:NRL1 V1 and TRV:NRL V2 N. benthamiana leaves. The silencing of NRL1 shows attenuation of Pi02860-mediated degradation of StSWAP70 compared with TRV:GFP. Free GFP specific to TRV: GFP-expressing plants are detected below 30 kDa, indicting GFP is not silenced. Expression of constructs in the leaves is indicated by a “+.” Protein size markers are indicated in kilodaltons, and protein loading is indicated by Ponceau stain. Immunoblots with anti-GFP show protein fusion of GFP-StSWAP70 (triangle) and the anti-cMYC shows protein fusion of cMYC-StNRL1 (star) and cMYC-Pi02860 (arrow) of the expected size.