Fig. 3.

Time courses of ALK and LTK receptor phosphorylation stimulated by AUG-α or AUG-α AD. Time courses of ALK (A) or LTK (B) phosphorylation in L6 cells stably expressing these receptors. Phosphorylation was induced by saturating levels (10 nM) of AUG-α or AUG-α AD. Cell lysates were subjected to immunoprecipitation (IP) by anti-ALK or anti-LTK antibodies, and phosphorylation was monitored by anti-pTyr antibodies (IB: anti-pTyr). Anti-ALK or anti-LTK antibodies were used as loading controls. Total cell lysates (TCL) and anti-phosphoMAPK or anti-MAPK antibodies were used to analyze MAPK phosphorylation in response to AUG-α or AUG-α AD stimulation at different time points.